Preparation of15N-labeled L-alanine by coupling the alanine dehydrogenase and alcohol dehydrogenase reactions
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چکیده
منابع مشابه
Expression of an L-alanine dehydrogenase gene in Zymomonas mobilis and excretion of L-alanine.
An approach to broaden the product range of the ethanologenic, gram-negative bacterium Zymomonas mobilis by means of genetic engineering is presented. Gene alaD for L-alanine dehydrogenase (EC 1.4.1.1.) from Bacillus sphaericus was cloned and introduced into Z. mobilis. Under the control of the strong promoter of the pyruvate decarboxylase (pdc) gene, the enzyme was expressed up to a specific a...
متن کاملPartial purification and properties of Halobacterium cutirubrum L-alanine dehydrogenase.
1. Halobacterium cutirubrum L-alanine dehydrogenase was purified approx. 100-fold. 2. It has a mol. wt. of 72 500, about one-third that of two well-studied alanine dehydrogenases from non-halophiles. 3. The activity of the enzyme increases with temperature up to 70 degrees C, but the protein itself is not thermostable. 4. In the reductive amination reaction, the enzyme is fully active in the pr...
متن کاملMarkerless mutagenesis in Methanococcus maripaludis demonstrates roles for alanine dehydrogenase, alanine racemase, and alanine permease.
Among the archaea, Methanococcus maripaludis has the unusual ability to use L- or D-alanine as a nitrogen source. To understand how this occurs, we tested the roles of three adjacent genes encoding homologs of alanine dehydrogenase, alanine racemase, and alanine permease. To produce mutations in these genes, we devised a method for markerless mutagenesis that builds on previously established ge...
متن کاملInterference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.
Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.
متن کاملThe inhibition of alanine dehydrogenase by metal chelates of tetracycline.
In a recent, communication (1) we described the purification and properties of a soluble alanine dehydrogenase isolated from cell-free extracts of the H37Ra strain of Mycobacterium tuberculosis. This enzyme catalyzes the diphosphopyridine nucleotidelinked oxidative deamination of L-alanine to pyruvate. During the study of this enzyme it was observed that Terramycinl inhibits the oxidative react...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80294-9